ATF-2 (Ab-112 or 94) antibody detects endogenous levels of total ATF-2 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human ATF-2 around the phosphorylation site of serine 112 or 94 (D-L-S-PP-L).
ATF2
Reactivity: Human
WB, IHC, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:500~1:1000 IHC: 1:50~1:100.
Restrictions
For Research Use only
Format
Liquid
Concentration
100ug/100ul
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Gupta, Campbell, Dérijard, Davis: "Transcription factor ATF2 regulation by the JNK signal transduction pathway." in: Science (New York, N.Y.), Vol. 267, Issue 5196, pp. 389-93, (1995) (PubMed).
Livingstone, Patel, Jones: "ATF-2 contains a phosphorylation-dependent transcriptional activation domain." in: The EMBO journal, Vol. 14, Issue 8, pp. 1785-97, (1995) (PubMed).
van Dam, Wilhelm, Herr, Steffen, Herrlich, Angel: "ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents." in: The EMBO journal, Vol. 14, Issue 8, pp. 1798-811, (1995) (PubMed).
Abdel-Hafiz, Heasley, Kyriakis, Avruch, Kroll, Johnson, Hoeffler: "Activating transcription factor-2 DNA-binding activity is stimulated by phosphorylation catalyzed by p42 and p54 microtubule-associated protein kinases." in: Molecular endocrinology (Baltimore, Md.), Vol. 6, Issue 12, pp. 2079-89, (1993) (PubMed).