ATF-2 (Ab-62 or 44) antibody detects endogenous levels of total ATF-2 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human ATF-2 around the phosphorylation site of serine 62 or 44 (N-D-SP-V-I).
ATF2
Reactivity: Human
WB, IHC, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:500~1:1000 IHC: 1:50~1:100.
Restrictions
For Research Use only
Format
Liquid
Concentration
100ug/100ul
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Sevilla, Santos, Vega, Lazo: "Human vaccinia-related kinase 1 (VRK1) activates the ATF2 transcriptional activity by novel phosphorylation on Thr-73 and Ser-62 and cooperates with JNK." in: The Journal of biological chemistry, Vol. 279, Issue 26, pp. 27458-65, (2004) (PubMed).
Gupta, Campbell, Dérijard, Davis: "Transcription factor ATF2 regulation by the JNK signal transduction pathway." in: Science (New York, N.Y.), Vol. 267, Issue 5196, pp. 389-93, (1995) (PubMed).
Sakurai, Maekawa, Sudo, Ishii, Kishimoto: "Phosphorylation of cAMP response element-binding protein, CRE-BP1, by cAMP-dependent protein kinase and protein kinase C." in: Biochemical and biophysical research communications, Vol. 181, Issue 2, pp. 629-35, (1992) (PubMed).