Goat anti-Rabbit IgG (F(ab')2 Region) Antibody (HRP)

Catalog No. ABIN965219

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: F(ab')2 Region

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)

Specificity: F(ab')2 Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed detects F(ab')2 fragments.

Characteristics: F(ab')2 fragments were generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab')2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab')2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab')2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining.
F(ab')2 Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Immunogen: Anti-Rabbit IgG F(ab')2 Antibody was produced in goat by repeated immunization with Rabbit IgG F(ab')2 fragment.
Immunogen Type: Native Protein

Application Details

Application Notes: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide!
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Rabbit F(ab')2 Antibody, Gt-a-Rabbit F(ab')2 conjugated, Rabbit F(ab')2 Peroxidase Antibody in goat, Rabbit F(ab')2 Peroxidase conjugated F(ab')2 Secondary Antibody.