Rabbit anti-Cat IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN965022

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Cat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Cat IgG and Cat Serum.

Characteristics: Anti-Rat IgG (H&L) antibody generated in rabbit detects specifically Cat IgG (H&L). This secondary antibody anti-Cat is ideal for investigators who routinely perform titration assays, westCrn-blot, immunoprecipitation and more generally immunoassays.
Anti-Rat IgG (H&L) antibody generated in rabbit detects specifically Cat IgG (H&L). This secondary antibody anti-Cat is ideal for investigators who routinely perform titration assays, westCrn-blot, immunoprecipitation and more generally immunoassays.

Purification: Preadsorption: Solid phase absorption

Immunogen: Anti-Cat IgG (H&L) was produced by repeated immunization with Cat IgG whole molecule in rabbit.
Immunogen Type: Native Protein

Application Details

Application Notes: Antibody anti-Cat has been assayed against 1.0 µg of Cat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. Antibody anti-Cat has been assayed against 1.0 µg of Cat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide!
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Cat F(ab')2 IgG (H&L) peroxidase conjugated Antibody, Rabbit-a-Cat F(ab')2 IgG (H&L), Cat F(ab')2 IgG (H&L) peroxidase conjugated Antibody in rabbit, Rabbit F(ab')2 IgG (H&L) peroxidase conjugated Secondary Antibody.