POLB antibody (C-Term)

Catalog No. ABIN964928

Supplier Product No.: 600-401-c65

Product Details anti-POLB Antibody

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-c65

Supplier: Rockland

Purpose: POLB Antibody

Cross-Reactivity (Details): This antibody reacts with overexpressed and endogenous POLß protein.

Characteristics: Synonyms: rabbit anti-POLB antibody, rabbit anti-DNA polymerase beta antibody, DNA polymerase ß, POLß, POL-B, POL-ß, pol-beta, beta-pol

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POLß protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified POLß antibody has been tested by ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kDa in size corresponding to POLß by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 0.5 μg/mL

ELISA Dilution: 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.4 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-POLB antibody (ABIN964928)

Asagoshi, Liu, Masaoka, Lan, Prasad, Horton, Brown, Wang, Bdour, Sobol, Taylor, Yasui, Wilson: "DNA polymerase beta-dependent long patch base excision repair in living cells." in: DNA repair, Vol. 9, Issue 2, pp. 109-19, (2010) (PubMed).

Sobol, Foley, Nyska, Davidson, Wilson: "Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice." in: DNA repair, Vol. 2, Issue 5, pp. 609-22, (2004) (PubMed).

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: POLB (POLB Products)

Background: Background: The POLß protein belongs to the DNA polymerase type-X family. It is a DNA polymerase involved in gap-filling DNA synthesis or base excision and repair in a stepwise distributive fashion unlike other DNA polymerases. It is required for DNA maintenance, replication, recombination, and drug resistance. It is normally found as a monomer, in the cytoplasm and it translocates to the nucleus upon DNA damage.

Gene ID: 5423

NCBI Accession: NP_002681

UniProt: P06746

Pathways: DNA Damage Repair