RRM2B antibody (N-Term)

Catalog No. ABIN964915

Product Details anti-RRM2B Antibody

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRM2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: Anti-Human RRM2B/p53R2 is directed against RRM2B and reacts with the RRM2B from human tissues. Based on sequence we expect this antibody to react as well with RRM2B from other species. Based on 100% homology to the immunizing sequence, one may expect reactivity to chimpanzee and orangutan, based on 93% homology, reactivity to macaque, and 92% to pig and Drosophila.

Characteristics: RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-iron-carboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT). Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.

Purification: affinity purified

Sterility: Sterile filtered

Immunogen: Anti-Human RRM2B/p53R2 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein. A residue of cysteine was added to facilitate coupling.
Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes: RRM2B/p53R2 antibody is suitable for IP, IHC, IF, ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RRM2B1 by western blotting in the appropriate cell lysate or extract.

Comment:

Gene Name: RRM2B

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.82 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References for anti-RRM2B antibody (ABIN964915)

Xue, Liu, Wang, Liu, Chen, Jia, Hsie, Chen, Luh, Zheng, Yen: "Ribonucleotide reductase subunit M2B deficiency leads to mitochondrial permeability transition pore opening and is associated with aggressive clinicopathologic manifestations of breast cancer." in: American journal of translational research, Vol. 10, Issue 11, pp. 3635-3649, (2018) (PubMed).

Chen, Tsou, Hu, Ma, Liu, Yen, Ann: "Autophagy induction causes a synthetic lethal sensitization to ribonucleotide reductase inhibition in breast cancer cells." in: Oncotarget, Vol. 7, Issue 2, pp. 1984-99, (2016) (PubMed).

Kuo, Sy, Xue, Chi, Lee, Yen, Chiang, Chang, Chu, Yen: "RRM2B suppresses activation of the oxidative stress pathway and is up-regulated by p53 during senescence." in: Scientific reports, Vol. 2, pp. 822, (2013) (PubMed).

Liu, Lai, Wang, Xue, Leora, Wu, Hu, Zhang, Kuo, Zhou, Zhang, Wang, Wang, Zhou, Nelson, Zheng, Zhang, Chu, Yen: "Ribonucleotide reductase small subunit M2B prognoses better survival in colorectal cancer." in: Cancer research, Vol. 71, Issue 9, pp. 3202-13, (2011) (PubMed).

Wang, Liu, Xue, Zhang, Kuo, Hu, Zhou, Ann, Zhang, Yen: "Ribonucleotide reductase subunit p53R2 regulates mitochondria homeostasis and function in KB and PC-3 cancer cells." in: Biochemical and biophysical research communications, Vol. 410, Issue 1, pp. 102-7, (2011) (PubMed).

Target Details for RRM2B

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Alternative Name: RRM2B p53R2 (RRM2B Products)

Synonyms: p53r2 antibody, RRM2B antibody, MTDPS8A antibody, MTDPS8B antibody, P53R2 antibody, p53R2 antibody, ribonucleotide reductase M2 B (TP53 inducible) S homeolog antibody, ribonucleotide reductase regulatory TP53 inducible subunit M2B antibody, ribonucleotide reductase M2 B (TP53 inducible) antibody, rrm2b.S antibody, RRM2B antibody, rrm2b antibody, Rrm2b antibody

Background: RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-iron-carboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT). Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.
Synonyms: Ribonucleoside-diphosphate reductase subunit M2 B; Ribonucleotide Reductase, R2/beta subunit; small subunit of p53 (MIM 191170)-inducible ribonucleotide reductase; RIR2B; RNRR2, TP53-inducible ribonucleotide reductase M2 B.

Gene ID: 50484, 42544136

UniProt: Q7LG56

Pathways: p53 Signaling, Negative Regulation of intrinsic apoptotic Signaling