IL-1 beta antibody

Catalog No. ABIN964782

Product Details anti-IL-1 beta Antibody

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Purpose: IL-1 Beta Antibody

Cross-Reactivity (Details): This antibody is primarily directed against mature, 17,000 MW mouse IL-1ß and is useful in determining its presence in various assays.

Characteristics: Synonyms: rabbit anti-IL-1 beta antibody, rabbit anti-IL-1b antibody, rabbit anti-Interleukin-1 beta antibody, IL-1ß, catabolin

Purification: This is an IgG preparation of whole rabbit serum purified by DEAE fractionation.

Immunogen:

Immunogen: This antibody was prepared by repeated immunizations with recombinant mouse IL-1ß produced in E.coli.  The MW of recombinant mouse IL-1ß was 17 kDa.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:50-1:250

Application Note: Anti-Mouse IL-1ß has been tested for use in immunohistochemistry, immunoblotting and immunofluorescence. This antibody is useful in ELISA, neutralizations, radioimmunoassays, flow cytometry, and immunoprecipitation. It recognizes the 17,000 MW mature IL-1ß. For immunoblots, typically, IL-1ß is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG (p/n 011-001-297) to reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL-1ß. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (611-1302) for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of mouse and rat IL-1ß activity in bioassays. It does not neutralize the biological activity IL-1α. It does not neutralize the biological activity of human or primate IL-1ß. For neutralization, it is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may interact with cells non-specifically.

Neutralization Dilution: User Optimized

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:200-1:800

ELISA Dilution: 1:1,000 - 1:5,000

IF Microscopy Dilution: 1:50-1:250

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-IL-1 beta antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

References for anti-IL-1 beta antibody (ABIN964782)

Rawji, Young, Ghosh, Michaels, Mirzaei, Kappen, Kolehmainen, Alaeiilkhchi, Lozinski, Mishra, Pu, Tang, Zein, Kaushik, Keough, Plemel, Calvert, Knights, Gaffney, Tetzlaff, Franklin, Yong: "Niacin-mediated rejuvenation of macrophage/microglia enhances remyelination of the aging central nervous system." in: Acta neuropathologica, Vol. 139, Issue 5, pp. 893-909, (2020) (PubMed).

Bouvier, Jones, Quesseveur, Davoli, A Ferreira, Quirion, Mechawar, Murai: "High Resolution Dissection of Reactive Glial Nets in Alzheimer's Disease." in: Scientific reports, Vol. 6, pp. 24544, (2017) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: Il1b (IL1B Products)

Background: Background: IL-1 beta (also known as Interleukin-1 beta, IL-1ß and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1ß is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Anti-IL-1 beta antibody is ideal for investigators involved in Cardiovascular and Immunology research.

Gene ID: 16176

UniProt: P10749

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome