CCL3 antibody

Catalog No. ABIN964781

Supplier Product No.: 210-401-315

Product Details anti-CCL3 Antibody

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 210-401-315

Supplier: Rockland

Purpose: MIP-1 alpha Antibody

Cross-Reactivity (Details): This antibody is specific for mouse MIP-1α protein.

Characteristics: Synonyms: rabbit anti-MIP-1α antibody, rabbit anti-MIP-1 alpha antibody, Mouse Macrophage Inflammatory Protein-1-alpha, Ccl3 cytokine, C-C motif chemokine 3, Small-inducible cytokine A3, Macrophage inflammatory protein 1-α, MIP-1-alpha, TY-5, SIS-alpha, Heparin-binding chemotaxis protein, L2G25B, Scya3 protein

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse MIP-1alpha protein produced in E.coli.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This purified antibody has been tested in western blotting and is suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7-8 kDa in size corresponding to the mature 92 amino acid mouse MIP-1α/Ccl3 protein by western blotting in appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: Ccl3 (CCL3 Products)

Background: Background: Mouse MIP-1α, or Ccl3, of the intercrine beta (chemokine CC family) is a secreted cytokine with inflammatory, pyrogenic and chemokinetic properties. It is expressed in lung, spleen, and pancreas, and has a potent chemotactic activity for eosinophils. Ccl3 binding to a high-affinity receptor activates calcium release in neutrophils.

Gene ID: 20302

NCBI Accession: NP_035467

UniProt: P10855

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy