IL-33 antibody

Catalog No. ABIN964749

Supplier Product No.: 209-401-b50

Product Details anti-IL-33 Antibody

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 209-401-b50

Supplier: Rockland

Purpose: IL-33 Antibody

Cross-Reactivity (Details): In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids.

Characteristics: Synonyms: rabbit anti-Interleukin-33 antibody, rabbit anti-IL-33 antibody, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV

Purification: This purified antibody has been heated to 56°C for 30 minutes.

Immunogen:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 18 kDa in size corresponding to mature human IL-32α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Neutralization Dilution: 1:400

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-IL-33 antibody (ABIN964749)

Hayakawa, Hayakawa, Matsuyama, Tamemoto, Okazaki, Tominaga: "Mature interleukin-33 is produced by calpain-mediated cleavage in vivo." in: Biochemical and biophysical research communications, Vol. 387, Issue 1, pp. 218-22, (2009) (PubMed).

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL33 (IL33 Products)

Background: Background: IL-33 (also known as Interleukin-33, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV) is a cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 induces T helper type 2-associated cytokines. IL-33 is a secreted cytokine that is expressed at high levels in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes and is almost undetectable in placenta. The 31 kDa precursor is proteolytically converted to an 18 kDa mature form by CASP1. Anti-IL-33 antibody is ideal for investigators involved in Cardiovascular and Immunology research.

Gene ID: 90865, 15559209

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response