OspB antibody

Catalog No. ABIN1043770

Supplier Product No.: 200-401-c15s

Product Details

Target: OspB

Reactivity: Borrelia burgdorferi

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 200-401-c15s

Supplier: Rockland

Purpose: OspB Antibody

Cross-Reactivity (Details): This antibody is specific for Borrelia burgdorferi OspB protein.

Characteristics: Synonyms: rabbit anti-OspB Antibody, Outer surface protein B, Borrelia burgdorferi OspB, locus BB_A16

Purification: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.

Immunogen:

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspB protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 30.3 kDa in size corresponding to Borrelia burgdorferi OspB protein by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:13,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Neelakanta, Li, Pal, Liu, Beck, DePonte, Fish, Kantor, Fikrig: "Outer surface protein B is critical for Borrelia burgdorferi adherence and survival within Ixodes ticks." in: PLoS pathogens, Vol. 3, Issue 3, pp. e33, (2007) (PubMed).

Liang, Caimano, Radolf, Fikrig: "Borrelia burgdorferi outer surface protein (osp) B expression independent of ospA." in: Microbial pathogenesis, Vol. 37, Issue 1, pp. 35-40, (2004) (PubMed).

Haake: "Spirochaetal lipoproteins and pathogenesis." in: Microbiology (Reading, England), Vol. 146 ( Pt 7), pp. 1491-504, (2000) (PubMed).

Target Details

Target: OspB

Background: Background: OspB, is one of the major Outer Surface Proteins of the outer membrane of Borrelia burgdorferi, which is composed of various unique outer surface proteins (Osp) that have been characterized (OspA through OspF). The Osp proteins are lipoproteins anchored by N-terminally attached fatty acid molecules to the membrane. They are presumed to play a role in virulence, transmission, or survival in the tick. Two of the major surface Ag of Borrelia burgdorferi, the 31- kDa OspA and 34- kDa OspB proteins, show a high degree of sequence similarity, are encoded by a 49-kb plasmid and share a common promoter, and are coordinately transcribed. OspA, OspB, and OspD are expressed by B. burgdorferi residing in the gut of unfed ticks, suggesting that they promote the persistence of the spirochete in ticks between blood meals. OspB has a contributing role in the adherence of B. burgdorferi to the tick gut. The C terminus of OspB is important for eliciting a protective immune response to OspB. B. burgdorferi has the ability to vary its surface proteins in response to immune attack.

Gene ID: 1194340

NCBI Accession: WP_010890379

UniProt: P17739