OspA antibody

Catalog No. ABIN964719

Supplier Product No.: 200-401-c13

Product Details anti-OspA Antibody

Target: OspA (Borrelia Burgdorferi Outer Surface Protein A (OspA))

Reactivity: Borrelia burgdorferi

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OspA antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 200-401-c13

Supplier: Rockland

Purpose: OspA Antibody

Cross-Reactivity (Details): This antibody is specific for Borrelia burgdorferi OspA protein.

Characteristics: Synonyms: rabbit anti-OspA Antibody, Outer surface protein A, Borrelia burgdorferi OspA, ospA, BB_A15, Plasmid lp54

Purification: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.

Immunogen:

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspA protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 28.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: >1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for OspA

Target: OspA (Borrelia Burgdorferi Outer Surface Protein A (OspA))

Alternative Name: ospA (OspA Products)

Background: Background: Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi encoded on its Plasmid lp54, is a major component of the spirochete's extracellular matrix. OspA probably serves as a lipid-anchor. The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression.

Gene ID: 1194357

NCBI Accession: NP_045688

UniProt: P0CL66