OspC antibody
Reactivity: Borrelia burgdorferi
ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
1 image
(4 references)-
- Target
- OspC
- Reactivity
- Borrelia burgdorferi
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB)
- Specificity
- This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspC protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi, afzelii, and valaisiana sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. japonica and americana sources based on 94% homology. Cross-reactivity with OspC from other sources has not been determined.
- Characteristics
- Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane.
- Purification
- purified
- Sterility
- Sterile filtered
- Immunogen
-
MBP-fusion protein corresponding to Borrelia burgdorferi OspC protein.
Immunogen Type: RecombinantProtein - Isotype
- IgG
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- Application Notes
- This protein-A purified antibody has been tested for use in Western blotting and in ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract.
- Comment
-
Gene Name: ospC, BB_B19
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- Expiry Date
- 12 months
-
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: "Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells." in: Microorganisms, Vol. 10, Issue 2, (2022) (PubMed).
: "Paper-based multiplexed vertical flow assay for point-of-care testing." in: Lab on a chip, Vol. 19, Issue 6, pp. 1027-1034, (2019) (PubMed).
: "Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival." in: Cellular microbiology, Vol. 19, Issue 12, (2018) (PubMed).
: "A short-term Borrelia burgdorferi infection model identifies tissue tropisms and bloodstream survival conferred by adhesion proteins." in: Infection and immunity, Vol. 83, Issue 8, pp. 3184-94, (2015) (PubMed).
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: "Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells." in: Microorganisms, Vol. 10, Issue 2, (2022) (PubMed).
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- Target
- OspC
- Background
-
Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane.
Synonyms: OspC, Borrelia burgdorferi OspC, PC, Outer Surface Protein C - Gene ID
- 1194415
- NCBI Accession
- NP_047005
- UniProt
- Q07337
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