VEGFA antibody

Catalog No. ABIN964713

Supplier Product No.: 200-401-b74

Product Details anti-VEGFA Antibody

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-401-b74

Supplier: Rockland

Purpose: VEGF-A Antibody

Cross-Reactivity (Details): This antibody is specific for bovine VEGF-A protein.

Characteristics: Synonyms: rabbit anti-VEGF-A Antibody, Vascular endothelial growth factor A, VEGF A, VEGFA, Vascular permeability factor, VPF

Purification: This product was Protein-A purified from monospecific antiserum by chromatography.

Immunogen:

Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 164 amino acids of the mature bovine VEGF-A protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.2 kDa in size corresponding to bovine VEGF-A protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:333

ELISA Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGFA (VEGFA Products)

Background: Background: VEGF is a potent mitogen in embryonic and somatic angiogenesis with specificity for vascular endothelial cells. VEGF forms homodimers and exists in four different isoforms. Overall, the VEGF monomer resembles that of PDGF, but its N-terminal segment is helical rather than extended. VEGF shares homologies of about 21 % and 24 % respectively with the A and B chains of human platelet-derived growth factor (PDGF), and has complete conservation of the eight cysteine residues found in both mature PDGF chains. The cysteine knot motif is a common feature of this domain. The homology is not reflected in function, however, since the cell types responsive to VEGF are distinct from those responsive to homo- and heterodimers of the PDGF chains. This protein is a glycosylated mitogen that acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. VEGF-A also has been shown to have effects on a number of other cell types (e.g. stimulation of monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells ). VEGF-A is also a vasodilator, it increases microvascular permeability, and was originally referred to as vascular permeability factor. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized.

Gene ID: 281572, 27806357

UniProt: P15691

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling