DYKDDDDK Tag antibody

Catalog No. ABIN964701

Product Details anti-DYKDDDDK Tag Antibody

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is un-conjugated

Application: Immunoprecipitation (IP)

Sequence: DYKDDDDK

Specificity: This product is a monoclonal antibody coupled to activated agarose. This antibody is directed against the DYKDDDDK epitope tag and is useful in determining its presence in over expressed proteins in various assays.  The antibody recognizes the DYKDDDDK epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Characteristics: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

Purification: purified

Sterility: Sterile filtered

Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the DYKDDDDK epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
Immunogen Type: Peptide

Clone: 29E4-G7

Isotype: IgG2a kappa

Application Details

Application Notes: Anti-DYKDDDDK Affinity Gel is optimally suited for immunoprecipitation and purification of DYKDDDDK tagged fusion proteins. Anti-DYKDDDDK Affinity Gel antibody recognizes the DYKDDDDK epitope tag fused to either the amino- or carboxy- terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) for competitive elution to recover fusion protein (see protocol). Refer to the Protocol for complete instructions for use including preferred buffers for elution. Do not use buffers that may denature the anti-DYKDDDDK antibody.

Comment:

1 mL agarose-conjugated antibody in 1.5 mL buffer

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Abstract: DYKDDDDK Tag Products

Target Type: Tag

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Anti-epitope tag antibodies serve as universal detection reagents for any tag containing protein produced by recombinant means.  Epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, DYKDDDDK and HA.
Synonyms: DYKDDDDK tag, DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, D-Y-K-D-D-D-D-K