Luciferase antibody

Catalog No. ABIN964679

Supplier Product No.: 200-101-c44

Product Details anti-Luciferase Antibody

Target: Luciferase

Reactivity: Photinus pyralis

Host: Goat

Clonality: Polyclonal

Conjugate: This Luciferase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 200-101-c44

Supplier: Rockland

Purpose: Luciferase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)].

Characteristics: Synonyms: goat anti-Luciferase Antibody, Luciferin 4-monooxygenase, Photinus pyralis (Common eastern firefly) (Lampyris pyralis)

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Luciferase [Photinus pyralis (Firefly)]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1500

Application Note: Anti-Luciferase Antibody has been tested in Western Blot, IHC, IF, and ELISA. Expect a band ~60 kDa in appropriate cell lysates. Although not tested, this antibody would be useful in immunoprecipitation, immunocytochemistry, and most immunological methods requiring high titers and specificity.

Western Blot Dilution: 1:1000 - 1:5000

ELISA Dilution: 1:200 - 1:1000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Luciferase antibody (ABIN964679)

Pommier, Anaparthy, Memos, Kelley, Gouronnec, Yan, Auffray, Albrengues, Egeblad, Iacobuzio-Donahue, Lyons, Fearon: "Unresolved endoplasmic reticulum stress engenders immune-resistant, latent pancreatic cancer metastases." in: Science (New York, N.Y.), Vol. 360, Issue 6394, (2018) (PubMed).

Kenny, Duval, Sammut, Steele, Pritchard, Atherton, Argent, Dimaline, Dockray, Varro: "Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 3, pp. G431-41, (2008) (PubMed).

Target Details for Luciferase

Target: Luciferase

Abstract: Luciferase Products

Background: Background: Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.