Protein A antibody

Catalog No. ABIN964678

Supplier Product No.: 200-101-077

Product Details anti-Protein A Antibody

Target: Protein A

Reactivity: Staphylococcus aureus

Host: Goat

Clonality: Polyclonal

Conjugate: This Protein A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Supplier Product No.: 200-101-077

Supplier: Rockland

Purpose: Protein A Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A  [Staphylococcus aureus] and anti-Goat Serum.

Characteristics: Synonyms: goat anti-Protein A Antibody, goat anti Protein A, goat anti PA

Purification: Protein A Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Protein A  [Staphylococcus aureus]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Protein A Antibody has been tested by western blot and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. Optimal concentration in immunoassays should be determined by the researcher.

Western Blot Dilution: 1:2,000 - 1:10,000

Immunoprecipitation Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Protein A antibody (ABIN964678)

Treise, Huber, Klein-Rodewald, Heinemeyer, Grassmann, Basler, Adler, Rathkolb, Helming, Andres, Klaften, Landbrecht, Wieland, Strom, McCoy, Macpherson, Wolf, Groettrup, Ollert, Neff, Gailus-Durner et al.: "Defective immuno- and thymoproteasome assembly causes severe immunodeficiency. ..." in: Scientific reports, Vol. 8, Issue 1, pp. 5975, (2018) (PubMed).

Target Details for Protein A

Target: Protein A

Abstract: Protein A Products

Background: Background: Anti-Protein A Antibody detects Protein A. Protein A is a 56 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus encoded by the spa gene. Protein A is an efficient binding protein to the immunoglobulins and is used in biochemical research because of its ability to bind. Protein A binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. It does not react with human IgG3 or IgD, nor will it react to mouse IgM, IgA or IgE. Protein A is commonly used in bioprocessing and bioseparations.