ZNF148 antibody

Catalog No. ABIN964672

Supplier Product No.: 100-401-685

Product Details anti-ZNF148 Antibody

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF148 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 100-401-685

Supplier: Rockland

Purpose: ZBP-89 Antibody

Cross-Reactivity (Details): This polyclonal antibody is specific for human ZBP-89.

Characteristics: Synonyms: rabbit anti-ZBP-89 Antibody, Transcription factor ZBP89 antibody, Zinc finger DNA binding protein 89 antibody, Zinc finger protein 148 antibody, ZNF 148 antibody, Zinc finger protein 148, Transcription factor ZBP-89, ZNF148

Purification: Anti-ZBP89 antibody was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: Purified full length ZBP-89 recombinant protein expressed in E.coli.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Application Note: This polyclonal antibody reacts with human ZBP-89 in a variety of tested immunological assays including western blot and ELISA.  Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:5,000 dilution is recommended. A band at approximately 89 kDa corresponding to human ZBP-89 is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937 cells can be used as a positive control. For ELISA a 1:10,000 to 1:30,000 dilution is recommended. Researchers should determine optimal titers for other applications.

Western Blot Dilution: 1:5,000

ELISA Dilution: 1:10,000 - 1:30,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 90 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store ZBP89 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-ZNF148 antibody (ABIN964672)

Ohneda, Ohmori, Ishijima, Nakano, Yamamoto: "Characterization of a functional ZBP-89 binding site that mediates Gata1 gene expression during hematopoietic development." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30187-99, (2009) (PubMed).

Guyot, Murai, Fujiwara, Valverde-Garduno, Hammett, Wells, Dear, Orkin, Porcher, Vyas: "Characterization of a megakaryocyte-specific enhancer of the key hemopoietic transcription factor GATA1." in: The Journal of biological chemistry, Vol. 281, Issue 19, pp. 13733-42, (2006) (PubMed).

Target Details for ZNF148

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Alternative Name: ZNF148 (ZNF148 Products)

Background: Background: The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The full-length protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.

Gene ID: 256711

UniProt: Q9UQR1