ESRP2 antibody

Catalog No. ABIN964643

Product Details anti-ESRP2 Antibody

Target: ESRP2 (Epithelial Splicing Regulatory Protein 2 (ESRP2))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ESRP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Esrp-2 Antibody

Cross-Reactivity (Details): This antibody reacts with mouse Esrp-2 protein.

Characteristics: Synonyms: mouse anti-Esrp2 antibody, mouse anti-Esrp-2 antibody, RNA-binding protein 35B, RNA-binding motif protein 35B

Purification: Anti-Esrp-2 is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.

Immunogen Type: Recombinant Protein

Clone: 22C5-H7-A6

Isotype: IgG2a kappa

Application Details

Application Notes:

Application Note: Anti-Esrp-2 protein A purified antibody has been tested for use western blotting and ELISA. This antibody shows specific reactivity by with ESRP2 transfected cell lysates.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-ESRP2 antibody (ABIN964643)

Heilmann, Toth, Bossmann, Klimo, Plass, Gerhauser: "Genome-wide screen for differentially methylated long noncoding RNAs identifies Esrp2 and lncRNA Esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer." in: Oncogene, Vol. 36, Issue 46, pp. 6446-6461, (2017) (PubMed).

Warzecha, Jiang, Amirikian, Dittmar, Lu, Shen, Guo, Xing, Carstens: "An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition." in: The EMBO journal, Vol. 29, Issue 19, pp. 3286-300, (2010) (PubMed).

Target Details for ESRP2

Target: ESRP2 (Epithelial Splicing Regulatory Protein 2 (ESRP2))

Alternative Name: Esrp2 (ESRP2 Products)

Background: Background: Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Gene ID: 77411

UniProt: Q8K0G8