ESRP1 antibody

Catalog No. ABIN964639

Product Details anti-ESRP1 Antibody

Target: ESRP1 (Epithelial Splicing Regulatory Protein 1 (ESRP1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ESRP1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with mouse Esrp-1 protein.  A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee .  The binding epitope of this monoclonal antibody has not been mapped.

Characteristics: Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Purification: purified

Sterility: Sterile filtered

Immunogen: Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
Immunogen Type: RecombinantProtein

Clone: 27H12-F3-H5

Isotype: IgG2b kappa

Application Details

Application Notes: This protein-A purified antibody has been tested for use western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 by western blotting in the appropriate cell lysate or extract.

Comment:

Gene Name: Esrp1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Expiry Date: 3 months

References for anti-ESRP1 antibody (ABIN964639)

Kim, Kim, Oh, Hong, Kim: "Pressure-overload cardiac hypertrophy is associated with distinct alternative splicing due to altered expression of splicing factors." in: Molecules and cells, Vol. 37, Issue 1, pp. 81-7, (2014) (PubMed).

Warzecha, Jiang, Amirikian, Dittmar, Lu, Shen, Guo, Xing, Carstens: "An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition." in: The EMBO journal, Vol. 29, Issue 19, pp. 3286-300, (2010) (PubMed).

Target Details for ESRP1

Target: ESRP1 (Epithelial Splicing Regulatory Protein 1 (ESRP1))

Alternative Name: Esrp-1 (ESRP1 Products)

Synonyms: rbm35a antibody, RBM35A antibody, RMB35A antibody, 2210008M09Rik antibody, A630065D16 antibody, BC031468 antibody, Rbm35a antibody, RGD1560481 antibody, wu:fi28a07 antibody, zgc:154050 antibody, epithelial splicing regulatory protein 1 antibody, epithelial splicing regulatory protein 1 L homeolog antibody, ESRP1 antibody, esrp1.L antibody, Esrp1 antibody, esrp1 antibody

Background: Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Synonyms: Rbm35a, Epithelial splicing regulatory protein 1, RNA-binding protein 35A, RNA-binding motif protein 35A.

Gene ID: 207920

NCBI Accession: NP_918944

UniProt: Q3US41