YBX3/DBPA antibody

Catalog No. ABIN964622

Product Details anti-YBX3/DBPA Antibody

Target: YBX3/DBPA (YBX3) (Y Box Binding Protein 3 (YBX3))

Reactivity: Borrelia burgdorferi

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YBX3/DBPA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: DbpA Antibody

Cross-Reactivity (Details): This antibody is specific for Borrelia burgdorferi DbpA protein.

Characteristics: Synonyms: rabbit anti-DbpA Antibody, Decorin-binding Protein, Borrelia burgdorferi DbpA, dbp-A, dbp A

Purification: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.

Immunogen:

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-A protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18.5 kDa in size corresponding to Borrelia burgdorferi DbpA protein by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for YBX3/DBPA

Target: YBX3/DBPA (YBX3) (Y Box Binding Protein 3 (YBX3))

Alternative Name: DBP A (YBX3 Products)

Background: Background: This product is antibody made against DbpA, or Decorin Binding Protein A from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. DbpA from other microbial organisms such as E. coli (ATP-dependent RNA helicase DbpA) are significantly different. The spirochete migrates from the tick midgut during tick feeding to tick salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix, enabling bacteria to colonize in mammalian tissues. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host.

Gene ID: 1194347

NCBI Accession: WP_010890380

UniProt: O50917