CASZ1 antibody (Internal Region)

Catalog No. ABIN964598

Supplier Product No.: 600-401-b62

Product Details anti-CASZ1 Antibody

Target: CASZ1 (Castor Zinc Finger 1 (CASZ1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASZ1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-b62

Supplier: Rockland

Purpose: CASZ1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human CASZ1 protein.

Characteristics: Synonyms: rabbit anti-CASZ1 Antibody, CASZ1, Zinc finger protein castor homolog 1, Castor-related protein, Zinc finger protein 693, CST, SRG, ZNF693

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Casz1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This antibody has been tested for use in ELISA, IHC, IF, and western blotting. This antiserum detects endogenous CASZ1 proteins, both the hCASZ5 and hCASZ11 isoforms. Expect a band approximately 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in size corresponding CASZ1 by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:45,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.09 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-CASZ1 antibody (ABIN964598)

Lunde, Okaty, Dymecki, Glover: "Molecular Profiling Defines Evolutionarily Conserved Transcription Factor Signatures of Major Vestibulospinal Neuron Groups." in: eNeuro, Vol. 6, Issue 1, (2019) (PubMed).

Zhang, Bonasio, Strino, Kluger, Holloway, Modzelewski, Cohen, Reinberg: "SFMBT1 functions with LSD1 to regulate expression of canonical histone genes and chromatin-related factors." in: Genes & development, Vol. 27, Issue 7, pp. 749-66, (2013) (PubMed).

Target Details for CASZ1

Target: CASZ1 (Castor Zinc Finger 1 (CASZ1))

Alternative Name: CASZ1 (CASZ1 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The human castor zinc finger 1 gene (CASZ1) regulates the production of the castor transcription factor, having increased expression when cells of neural and mesenchymal origin are induced to differentiation. CASZ1 is expressed in a number of human tumors and it localizes to a genomic region frequently lost in tumors of neuroectodermal origin. The CASZ1 gene generates 2 mRNA isoforms hCASZ5 and hCASZ11 that could encode proteins containing 5 or 11 zinc finger motifs, respectively. The proteins may be especially labile. Expect a band at 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in fresh lysates.

Gene ID: 54897, 145207289

UniProt: Q86V15