CASZ1 antibody (Internal Region)

Catalog No. ABIN964598

Product Details anti-CASZ1 Antibody

Target: CASZ1 (Castor Zinc Finger 1 (CASZ1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Chimpanzee, Drosophila melanogaster, Macaque

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASZ1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This affinity purified antibody is directed against human CASZ1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with CASZ1 proteins from human, mouse, Drosophila, chimpanzee, and macaque based on a 100% homology. Partial reactivity is expected with horse and dog CASZ1 based on a 92% homology with the immunizing sequence. Cross-reactivity with CASZ1 from other sources has not been determined.

Cross-Reactivity: Mouse (Murine), Fruit Fly (Drosophila melanogaster), Chimpanzee, Macaque

Characteristics: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The human castor zinc finger 1 gene (CASZ1) regulates the production of the castor transcription factor, having increased expression when cells of neural and mesenchymal origin are induced to differentiation. CASZ1 is expressed in a number of human tumors and it localizes to a genomic region frequently lost in tumors of neuroectodermal origin. The CASZ1 gene generates 2 mRNA isoforms hCASZ5 and hCASZ11 that could encode proteins containing 5 or 11 zinc finger motifs, respectively. The proteins may be especially labile. Expect a band at 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in fresh lysates.

Purification: affinity purified

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Casz1 protein.
Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes: This antibody has been tested for use in ELISA and western blotting. This antiserum detects endogenous CASZ1 proteins, both the hCASZ5 and hCASZ11 isoforms. Expect a band approximately 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in size corresponding CASZ1 by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Comment:

Gene Name: CASZ1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.09 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 ° C prior to restoration. For extended storage aliquot contents and freeze at -24 ° C or below. This product is stable for several weeks at 4 ° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References for anti-CASZ1 antibody (ABIN964598)

Lunde, Okaty, Dymecki, Glover: "Molecular Profiling Defines Evolutionarily Conserved Transcription Factor Signatures of Major Vestibulospinal Neuron Groups." in: eNeuro, Vol. 6, Issue 1, (2019) (PubMed).

Zhang, Bonasio, Strino, Kluger, Holloway, Modzelewski, Cohen, Reinberg: "SFMBT1 functions with LSD1 to regulate expression of canonical histone genes and chromatin-related factors." in: Genes & development, Vol. 27, Issue 7, pp. 749-66, (2013) (PubMed).

Target Details for CASZ1

Target: CASZ1 (Castor Zinc Finger 1 (CASZ1))

Alternative Name: CASZ1 (CASZ1 Products)

Synonyms: sb:eu580 antibody, wu:fk85f10 antibody, csta antibody, castor antibody, CAS11 antibody, CST antibody, SRG antibody, ZNF693 antibody, dJ734G22.1 antibody, 2410019P08Rik antibody, AV096627 antibody, BC040081 antibody, Cst antibody, D4Ertd432e antibody, RGD1563533 antibody, castor zinc finger 1 antibody, castor zinc finger 1 L homeolog antibody, CASZ1 antibody, casz1 antibody, casz1.L antibody, Casz1 antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. The human castor zinc finger 1 gene (CASZ1) regulates the production of the castor transcription factor, having increased expression when cells of neural and mesenchymal origin are induced to differentiation. CASZ1 is expressed in a number of human tumors and it localizes to a genomic region frequently lost in tumors of neuroectodermal origin. The CASZ1 gene generates 2 mRNA isoforms hCASZ5 and hCASZ11 that could encode proteins containing 5 or 11 zinc finger motifs, respectively. The proteins may be especially labile. Expect a band at 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in fresh lysates.
Synonyms: CASZ1, Zinc finger protein castor homolog 1, Castor-related protein, Zinc finger protein 693

Gene ID: 54897, 145207289

UniProt: Q86V15