hCAS5 (N-Term) antibody

Catalog No. ABIN964597

Supplier Product No.: 600-401-948

Product Details

Target: hCAS5

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 600-401-948

Supplier: Rockland

Purpose: Cas5 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human Cas5 protein.

Characteristics: Synonyms: rabbit anti-Cas5 antibody, Castor homolog 1 zinc finger protein, CST, SRG, ZNF693, CASZ1, Cas-5, Cas 5

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 125 kDa in size corresponding to hCas5 protein is expected in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.3 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: hCAS5

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. hCas5 (Castor) is the human homolog of a Drosophila gene that regulates neurogenesis. In human neuro-blastoma tumors where a loss of heterozygosity of CASZ1 is noted, hCas functions to suppress the tumorigenicity of cells. hCas5 is a developmentally regulated transcription factor found in neural and muscle cells. hCas5 is a Zn finger transcription factor that is predominantly found in the nucleus. This antibody is designed to facilitate the functional study of this protein and its role in developmental regulation and expression in human tumor tissues.

Gene ID: 54897

NCBI Accession: XP_016857029

UniProt: B3KRV8