Biotin antibody

Catalog No. ABIN964580

Supplier Product No.: 600-401-098

Product Details anti-Biotin Antibody

Target: Biotin

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Biotin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-098

Supplier: Rockland

Purpose: Biotin Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Characteristics: Synonyms: rabbit anti Biotin Antibody, rabbit anti-biotin Antibody

Purification: Biotin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Biotin Antibody has been tested by ELISA and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:200,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.02 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: This product is stable for several weeks at 4° C as an undiluted liquid. Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-Biotin antibody (ABIN964580)

Goulielmaki, Ioannidou, Tsekrekou, Stratigi, Poutakidou, Gkirtzimanaki, Aivaliotis, Evangelou, Topalis, Altmüller, Gorgoulis, Chatzinikolaou, Garinis: "Tissue-infiltrating macrophages mediate an exosome-based metabolic reprogramming upon DNA damage." in: Nature communications, Vol. 11, Issue 1, pp. 42, (2020) (PubMed).

Target Details for Biotin

Target: Biotin

Abstract: Biotin Products

Target Type: Chemical

Background: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.