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Biotin antibody

Rockland 600-401-098 WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN964580
Supplier Product No.: 600-401-098
  • Target See all Biotin products
    Biotin
    Reactivity
    Please inquire
    Host
    • 63
    • 35
    • 28
    Rabbit
    Clonality
    • 81
    • 37
    • 1
    Polyclonal
    Conjugate
    • 31
    • 13
    • 8
    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Biotin antibody is un-conjugated
    Application
    • 75
    • 52
    • 50
    • 46
    • 39
    • 32
    • 16
    • 13
    • 11
    • 9
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    600-401-098
    Supplier
    Rockland
    Purpose
    Biotin Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
    Characteristics
    Synonyms: rabbit anti Biotin Antibody, rabbit anti-biotin Antibody
    Purification
    Biotin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

    Immunogen Type: Other

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-Biotin Antibody has been tested by ELISA and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

    Western Blot Dilution: 1:2,000 - 1:10,000

    ELISA Dilution: 1:200,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.02 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    This product is stable for several weeks at 4° C as an undiluted liquid. Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Goulielmaki, Ioannidou, Tsekrekou, Stratigi, Poutakidou, Gkirtzimanaki, Aivaliotis, Evangelou, Topalis, Altmüller, Gorgoulis, Chatzinikolaou, Garinis: "Tissue-infiltrating macrophages mediate an exosome-based metabolic reprogramming upon DNA damage." in: Nature communications, Vol. 11, Issue 1, pp. 42, (2020) (PubMed).

  • Target
    Biotin
    Abstract
    Biotin Products
    Target Type
    Chemical
    Background
    Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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