ATG13 antibody (Internal Region, pSer318)

Catalog No. ABIN964569

Product Details anti-ATG13 Antibody

Target: ATG13 (Autophagy Related 13 (ATG13))

Binding Specificity: Internal Region, pSer318

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This affinity-purified antibody is directed against the phosphorylated form of human ATG13 protein at the pS318 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.  Reactivity occurs against human ATG13 pS318 protein and the antibody is specific for the phosphorylated form of the protein.   Reactivity with non-phosphorylated human ATG13 is minimal by ELISA and western blot.  A BLAST analysis was used to suggest cross reactivity with ATG13 from human based on 100% sequence homology with the immunogen.  Reactivity against homologues from other sources is not known.

Characteristics: ATG13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1 through their stable complex, and the regulation of ATG13-ULK1-RB1CC1. ATG13 also forms a stable complex with FIP200. Ulk1 phosphorylates ATG13 on S318 and promotes its release to damaged mitochondria. Autophagy is a normal process in eukaryotes required for turnover of cellular components during starvation and stress. It plays an essential role in cellular differentiation, cell death and aging. Defects in this evolutionarily conserved process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. ATG13 is one of several ATG genes required for autophagosome formation in mammalian cells. mTOR interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1.

Purification: affinity purified

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region near S318 of ATG13.
Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 56.6 kDa in size corresponding to human phosphorylated ATG13 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. 

Comment:

Gene Name: ATG13

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References for anti-ATG13 antibody (ABIN964569)

Jia, Bonifacino: "Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3." in: eLife, Vol. 8, (2020) (PubMed).

Izumi, Li, Shibaki, Mori, Yasunami, Sato, Matsunaga, Mae, Kodama, Kamijo, Kaneko, Nakagawara: "Recycling endosomal CD133 functions as an inhibitor of autophagy at the pericentrosomal region." in: Scientific reports, Vol. 9, Issue 1, pp. 2236, (2019) (PubMed).

Wang, Maxwell, Joo, Gwon, Messing, Mishra, Shaw, Ward, Quan, Sakurada, Pruett-Miller, Bertorini, Vogel, Kim, Peng, Taylor, Kundu: "ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97." in: Molecular cell, Vol. 74, Issue 4, pp. 742-757.e8, (2019) (PubMed).

Wallot-Hieke, Verma, Schlütermann, Berleth, Deitersen, Böhler, Stuhldreier, Wu, Seggewiß, Peter, Gohlke, Mizushima, Stork: "Systematic analysis of ATG13 domain requirements for autophagy induction." in: Autophagy, Vol. 14, Issue 5, pp. 743-763, (2019) (PubMed).

Thayer, Awad, Hegdekar, Sarkar, Tesfay, Burt, Zeng, Feldman, Lipinski: "The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability." in: Autophagy, pp. 1-14, (2019) (PubMed).

Fuqua, Mere, Kronemberger, Blomme, Bae, Turner, Harris, Scudese, Edwards, Ebert, de Sousa, Bodine, Yang, Adams, Lira: "ULK2 is essential for degradation of ubiquitinated protein aggregates and homeostasis in skeletal muscle." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, Issue 11, pp. 11735-11745, (2019) (PubMed).

Jeong, Simoneschi, Keegan, Melville, Adler, Saraf, Florens, Washburn, Cavasotto, Fenyö, Cuervo, Rossi, Pagano: "The ULK1-FBXW5-SEC23B nexus controls autophagy." in: eLife, Vol. 7, (2019) (PubMed).

Wang, Wang, Zhang, Luo, Liu, Xu, Diao, Liao, Liu: "Phosphorylation of ULK1 affects autophagosome fusion and links chaperone-mediated autophagy to macroautophagy." in: Nature communications, Vol. 9, Issue 1, pp. 3492, (2018) (PubMed).

Baron, Boudi, Dias, Schilling, Nölle, Vizcay-Barrena, Rattray, Jungbluth, Scheper, Fleck, Bates, Fanto: "Stall in Canonical Autophagy-Lysosome Pathways Prompts Nucleophagy-Based Nuclear Breakdown in Neurodegeneration." in: Current biology : CB, Vol. 27, Issue 23, pp. 3626-3642.e6, (2018) (PubMed).

Jung, Nayak, Schaeffer, Starzetz, Kirsch, Müller, Dikic, Mittelbronn, Behrends: "Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator." in: eLife, Vol. 6, (2017) (PubMed).

Hieke, Löffler, Kaizuka, Berleth, Böhler, Drießen, Stuhldreier, Friesen, Assani, Schmitz, Peter, Diedrich, Dengjel, Holland, Simonsen, Wesselborg, Mizushima, Stork: "Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells." in: Autophagy, Vol. 11, Issue 9, pp. 1471-83, (2016) (PubMed).

Puente, Hendrickson, Jiang: "Nutrient-regulated Phosphorylation of ATG13 Inhibits Starvation-induced Autophagy." in: The Journal of biological chemistry, Vol. 291, Issue 11, pp. 6026-35, (2016) (PubMed).

Park, Jung, Seo, Otto, Grunwald, Kim, Moriarity, Kim, Starker, Nho, Voytas, Kim: "The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14." in: Autophagy, Vol. 12, Issue 3, pp. 547-64, (2016) (PubMed).

Jiao, Su, Dong, Zhang, Xie, Yao, Chen, Wang, Liou, You: "Chaperone-like protein p32 regulates ULK1 stability and autophagy." in: Cell death and differentiation, Vol. 22, Issue 11, pp. 1812-23, (2016) (PubMed).

Rosenberg, Lafitte, Grant, Chen, Cleveland, Duckett: "Development of an HTS-Compatible Assay for the Discovery of Ulk1 Inhibitors." in: Journal of biomolecular screening, Vol. 20, Issue 7, pp. 913-20, (2016) (PubMed).

Egan, Chun, Vamos, Zou, Rong, Miller, Lou, Raveendra-Panickar, Yang, Sheffler, Teriete, Asara, Turk, Cosford, Shaw: "Small Molecule Inhibition of the Autophagy Kinase ULK1 and Identification of ULK1 Substrates." in: Molecular cell, Vol. 59, Issue 2, pp. 285-97, (2015) (PubMed).

Puustinen, Rytter, Mortensen, Kohonen, Moreira, Jäättelä: "CIP2A oncoprotein controls cell growth and autophagy through mTORC1 activation." in: The Journal of cell biology, Vol. 204, Issue 5, pp. 713-27, (2014) (PubMed).

Stanton, Dutta, Zhang, Polavaram, Leontovich, Hönscheid, Sinicrope, Tindall, Muders, Datta: "Autophagy control by the VEGF-C/NRP-2 axis in cancer and its implication for treatment resistance." in: Cancer research, Vol. 73, Issue 1, pp. 160-71, (2013) (PubMed).

Nazio, Strappazzon, Antonioli, Bielli, Cianfanelli, Bordi, Gretzmeier, Dengjel, Piacentini, Fimia, Cecconi: "mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6." in: Nature cell biology, Vol. 15, Issue 4, pp. 406-16, (2013) (PubMed).

Joo, Dorsey, Joshi, Hennessy-Walters, Rose, McCastlain, Zhang, Iyengar, Jung, Suen, Steeves, Yang, Prater, Kim, Thompson, Youle, Ney, Cleveland, Kundu: "Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy." in: Molecular cell, Vol. 43, Issue 4, pp. 572-85, (2011) (PubMed).

Target Details for ATG13

Target: ATG13 (Autophagy Related 13 (ATG13))

Alternative Name: ATG13 (ATG13 Products)

Synonyms: zgc:63526 antibody, KIAA0652 antibody, PARATARG8 antibody, 1110053A20Rik antibody, D2Ertd391e antibody, Harbi1 antibody, Harbi1l antibody, RGD1310685 antibody, autophagy related 13 antibody, ATG13 autophagy related 13 homolog (S. cerevisiae) antibody, ATG13 antibody, atg13 antibody, Atg13 antibody

Background: ATG13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1 through their stable complex, and the regulation of ATG13-ULK1-RB1CC1. ATG13 also forms a stable complex with FIP200. Ulk1 phosphorylates ATG13 on S318 and promotes its release to damaged mitochondria. Autophagy is a normal process in eukaryotes required for turnover of cellular components during starvation and stress. It plays an essential role in cellular differentiation, cell death and aging. Defects in this evolutionarily conserved process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. ATG13 is one of several ATG genes required for autophagosome formation in mammalian cells. mTOR interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1.
Synonyms: Autophagy-related protein 13, KIAA0652

Gene ID: 9776

NCBI Accession: NP_001136145

UniProt: O75143

Pathways: PI3K-Akt Signaling, Autophagy