His Tag antibody

Catalog No. ABIN964552

Supplier Product No.: 200-301-382

Product Details anti-His Tag Antibody

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 200-301-382

Supplier: Rockland

Purpose: 6X His Epitope Tag Antibody

Sequence: HHHHHH

Cross-Reactivity (Details): This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

Characteristics: Synonyms: mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Purification: 6X HIS Epitope Tag antibody was purified from concentrated tissue culture supernate by Protein A chromatography and is directed against the 6X His motif and is useful in determining its presence in various assays.

Sterility: Sterile filtered

Immunogen:

Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Immunogen Type: Conjugated Peptide

Clone: 33D10-D2-G8

Isotype: IgG1 kappa

Product Specific Information:

What can the His-tag antibody ABIN964552 be used for? This His-tag antibody is a monoclonal, unconjugated antibody for the specific detection of fusion proteins containing either amino- or carboxy-terminal His-epitope tags. It is suitable for the detection of His-tag in ELISA and Western blot, IHC and IP experiments.

What validation data is available for this His-tag antibody? The His-tag antibody has been used in 4 publications to date, which can be viewed at Pubmed. Four images are available for validation of the antibody in western blotting and ELISA.

What is the function of His-Tag? His-tag is one of the most commonly used tags. It is a series of six to nine histidine residues fused to either the amino or carboxyl terminus of a recombinant protein. His-tagging is particularly useful because it allows easy purification and detection of the target protein. The small size of the His-tag compared to other common epitope tags makes it less likely to interfere with the structure or function of the target protein. Consequently, it is well suited for use under denaturing conditions. Use our high-quality His-tag antibody to reliably detect His-tag.

Application Details

Application Notes:

Application Note: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, Anti-6X His/6XHIS can be used to identify fusion proteins that contain the 6X His epitope.  The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins.  This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Western Blot Dilution: 1:1000

Immunoprecipitation Dilution: 1:200

ELISA Dilution: 1:75,000

Other: FLUORESCENT POLARIZATION: 2 - 20 nMFRET: 2 - 20 nM

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 3.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store 6X HIS Tag Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-6X HIS Tag is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-His Tag antibody (ABIN964552)

Zhou, Pu, Chen, Hao, Gao, Ali, Hsiao, Stock, Goulian, Zhu: "Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens." in: Cell reports, Vol. 37, Issue 12, pp. 110147, (2022) (PubMed).

Vela-Corcía, Romero, de Vicente, Pérez-García: "Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance." in: Scientific reports, Vol. 8, Issue 1, pp. 7161, (2019) (PubMed).

Chavda, Ling, Majernick, Planey: "Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain." in: BMC biochemistry, Vol. 18, Issue 1, pp. 13, (2017) (PubMed).

Ramón-García, Ng, Jensen, Dosanjh, Burian, Morris, Folcher, Eltis, Grzesiek, Nguyen, Thompson: "WhiB7, an Fe-S-dependent transcription factor that activates species-specific repertoires of drug resistance determinants in actinobacteria." in: The Journal of biological chemistry, Vol. 288, Issue 48, pp. 34514-28, (2014) (PubMed).

Target Details for His Tag

Target: His Tag

Alternative Name: 6X His (His Tag Products)

Target Type: Tag

Background: Background: 6X His Tag Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.