Protein C Tag antibody

Catalog No. ABIN962838

Product Details anti-Protein C Tag Antibody

Target: Protein C Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This Protein C Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Brand: THE™

Specificity: THE^TM Protein C Tag Antibody, mAb, Mouse recognizes N-terminal and C-terminal Protein C tagged fusion proteins.

Characteristics: The THE^TM Protein C Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with Protein C tag peptide conjugated to KLH.

Purification: Protein A affinity column

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-0.5 µg/mL.
Immunoprecipitation: 1-5 µg/mL.
Flow cytometry: 1-3 µg for 1 x 10^6 cells.
ICC/IF: 1-3 µg/mL.
ELISA: 0.001-0.002 µg/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: THE^TM Protein C Tag Antibody, mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.

Expiry Date: 12 months

Target Details for Protein C Tag

Target: Protein C Tag

Abstract: Protein C Tag Products

Target Type: Tag

Background: Protein C is a Vitamin K-dependent plasma zymogen that is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme. Protein C Tag is a 12 amino acid sequence EDQVDPRLIDGK, which encodes residues 6 through 17 of the heavy chain of the protein. Protein C Tag Antibody is a useful tool in analysis of Protein C fusion proteins.