Strep Tag II antibody (Biotin)

Catalog No. ABIN962829

Product Details

Target: Strep Tag II

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Western Blotting (WB), ELISA

Brand: THE™

Sequence: NWSHPQFEK

Specificity: THE^TM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.

Characteristics: The THE^TM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse is THE^TM NWSHPQFEK Tag Antibody conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK (Strep II tag) tagged fusion proteins.

Purification: Protein A affinity column

Immunogen: NWSHPQFEK peptide conjugated - KLH

Clone: 5A9F9

Isotype: IgG1

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL
ELISA: 0.001-0.002 µg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

Buffer: PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Target Details

Target: Strep Tag II

Target Type: Tag

Background: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep II tag) is a nine amino acid peptide with high specificity and affinity towards streptavidin.