Strep Tag II antibody

Catalog No. ABIN962826

Product Details

Target: Strep Tag II

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Brand: THE™

Sequence: NWSHPQFEK

Specificity: THE^TM NWSHPQFEK Tag Antibody, mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.

Purification: Protein A affinity column

Immunogen: NWSHPQFEK peptide conjugated - KLH

Clone: 5A9F9

Isotype: IgG1

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-0.5 µg/mL
Immunoprecipitation: 1-5 µg/mL FACS: 1-3 µg for 1 x 10^6 cellsICC/IF: 1-3 µg/mL ELISA: 0.001-0.002 µg/mL Fusion Partner Fusion of SP2/0-Ag14 myeloma and B-lymphocytes

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

References

Duanmu, Casero, Dent, Gallaher, Yang, Rockwell, Martin, Pellegrini, Niyogi, Merchant, Grossman, Lagarias: "Retrograde bilin signaling enables Chlamydomonas greening and phototrophic survival." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 9, pp. 3621-6, (2013) (PubMed).

Piuri, Rondón, Urdániz, Hatfull: "Generation of affinity-tagged fluoromycobacteriophages by mixed assembly of phage capsids." in: Applied and environmental microbiology, Vol. 79, Issue 18, pp. 5608-15, (2013) (PubMed).

Avril, Tripathi, Brazier, Andisi, Janes, Soma, Sullivan, Bull, Stins, Smith: "A restricted subset of var genes mediates adherence of Plasmodium falciparum-infected erythrocytes to brain endothelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 26, pp. E1782-90, (2012) (PubMed).

Shanmugabalaji, Besagni, Piller, Douet, Ruf, Bock, Kessler: "Dual targeting of a mature plastoglobulin/fibrillin fusion protein to chloroplast plastoglobules and thylakoids in transplastomic tobacco plants." in: Plant molecular biology, Vol. 81, Issue 1-2, pp. 13-25, (2012) (PubMed).

Koprowski, Grajkowski, Isacoff, Kubalski: "Genetic screen for potassium leaky small mechanosensitive channels (MscS) in Escherichia coli: recognition of cytoplasmic ? domain as a new gating element." in: The Journal of biological chemistry, Vol. 286, Issue 1, pp. 877-88, (2011) (PubMed).

Olinares, Kim, Davis, van Wijk: "Subunit stoichiometry, evolution, and functional implications of an asymmetric plant plastid ClpP/R protease complex in Arabidopsis." in: The Plant cell, Vol. 23, Issue 6, pp. 2348-61, (2011) (PubMed).

Kolesi?ski, Piechota, Szczepaniak: "Initial characteristics of RbcX proteins from Arabidopsis thaliana." in: Plant molecular biology, Vol. 77, Issue 4-5, pp. 447-59, (2011) (PubMed).

Ueki, Spektor, Natale, Citovsky: "ANK, a host cytoplasmic receptor for the Tobacco mosaic virus cell-to-cell movement protein, facilitates intercellular transport through plasmodesmata." in: PLoS pathogens, Vol. 6, Issue 11, pp. e1001201, (2010) (PubMed).

Target Details

Target: Strep Tag II

Target Type: Tag

Background: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidins.