Myc Tag antibody

Catalog No. ABIN962814

Product Details anti-Myc Tag Antibody

Target: Myc Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myc Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF)

Brand: THE™

Specificity: THE^TM c-Myc Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc protein in human cancers or cell lines such as Hela cell lysates.

Characteristics: This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.

Purification: Protein A affinity column

Immunogen: A synthetic peptide EQKLISEEDL coupled - KLH

Clone: 2G8D5

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05 µg/mL-0.2 µg/mL
Western blot: 0.1 µg/mL-1 µg/mL
Western blot using ONE-HOUR Western Kit: For quick results, ONE-HOUR Western Kit L00205C is recommended. 0.9 µg of this antibody is mixed with diluted WB solution (dilute 1.5 mL WB with 1.5 mL of PBST).Immunoprecipitation (IP): 1 µg/mL
Immunofluorescent staining: 1 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5 mg/mL.

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

References for anti-Myc Tag antibody (ABIN962814)

Hyun, Lee, Rim, Kumar, Han, Lee, Lee, Kim: "De-novo RNA sequencing and metabolite profiling to identify genes involved in anthocyanin biosynthesis in Korean black raspberry (Rubus coreanus Miquel)." in: PLoS ONE, Vol. 9, Issue 2, pp. e88292, (2014) (PubMed).

Ridley, Thur, Shanahan, Thillaiappan, Shen, Uhl, Walden, Rahim, Waddington, Platt, van der Spoel: "?-Glucosidase 2 (GBA2) activity and imino sugar pharmacology." in: The Journal of biological chemistry, Vol. 288, Issue 36, pp. 26052-66, (2013) (PubMed).

Campos, Chapman, Deymier, Bronnimann, Ozbun: "Opposing effects of bacitracin on human papillomavirus type 16 infection: enhancement of binding and entry and inhibition of endosomal penetration." in: Journal of virology, Vol. 86, Issue 8, pp. 4169-81, (2012) (PubMed).

Zhu, Yu, Jiang, Gu: "Investigation of the influence of Arg555Trp and Thr538Pro TGFBI mutations on C-terminal cleavage and cell endoplasmic reticulum stress." in: Molecular vision, Vol. 18, pp. 1156-64, (2012) (PubMed).

Koo, Gang: "Suites of terpene synthases explain differential terpenoid production in ginger and turmeric tissues." in: PLoS ONE, Vol. 7, Issue 12, pp. e51481, (2012) (PubMed).

Stringer, Piper: "A single ubiquitin is sufficient for cargo protein entry into MVBs in the absence of ESCRT ubiquitination." in: The Journal of cell biology, Vol. 192, Issue 2, pp. 229-42, (2011) (PubMed).

Markus, Plevock, St Germain, Punch, Meaden, Lee: "Quantitative analysis of Pac1/LIS1-mediated dynein targeting: Implications for regulation of dynein activity in budding yeast." in: Cytoskeleton (Hoboken, N.J.), Vol. 68, Issue 3, pp. 157-74, (2011) (PubMed).

Faradji, Bloyer, Dardalhon-Cuménal, Randsholt, Peronnet: "Drosophila melanogaster Cyclin G coordinates cell growth and cell proliferation." in: Cell cycle (Georgetown, Tex.), Vol. 10, Issue 5, pp. 805-18, (2011) (PubMed).

Teterina, Pinto, Weaver, Jensen, Ehrenfeld: "Analysis of poliovirus protein 3A interactions with viral and cellular proteins in infected cells." in: Journal of virology, Vol. 85, Issue 9, pp. 4284-96, (2011) (PubMed).

Target Details for Myc Tag

Target: Myc Tag

Alternative Name: C-Myc Tag (Myc Tag Products)

Target Type: Tag

Background: The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen. c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins.