alpha Tubulin antibody (C-Term, Subunit alpha)
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- Target See all alpha Tubulin (TUBA1) Antibodies
- alpha Tubulin (TUBA1)
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Binding Specificity
- C-Term, Subunit alpha
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Reactivity
- Human, Saccharomyces cerevisiae, Mammalian, Avian, Echinodermata
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This alpha Tubulin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)
- Brand
- IHC-plus™
- Specificity
- Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.
- Purification
- Affinity purified
- Immunogen
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Yeast tubulin.
Type of Immunogen: Purified protein - Clone
- YL1-2
- Isotype
- IgG2a
- Top Product
- Discover our top product TUBA1 Primary Antibody
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- Application Notes
- Approved: ELISA (1:100 - 1:1000), IHC, IHC-Fr, IHC-P (5 μg/mL), IP, RIA, WB
- Comment
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Target Species of Antibody: Yeast
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- alpha Tubulin (TUBA1)
- Alternative Name
- Alpha Tubulin (TUBA1 Products)
- Pathways
- Microtubule Dynamics
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