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alpha Tubulin antibody (C-Term, Subunit alpha)

TUBA1 Reactivity: Human, Saccharomyces cerevisiae, Mammalian, Avian, Echinodermata WB, ELISA, IHC, IHC (p), IP, IHC (fro), RIA Host: Rat Monoclonal YL1-2 unconjugated
Catalog No. ABIN962076
  • Target See all alpha Tubulin (TUBA1) Antibodies
    alpha Tubulin (TUBA1)
    Binding Specificity
    • 9
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term, Subunit alpha
    Reactivity
    • 93
    • 70
    • 60
    • 24
    • 21
    • 16
    • 14
    • 14
    • 8
    • 7
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Saccharomyces cerevisiae, Mammalian, Avian, Echinodermata
    Host
    • 53
    • 44
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    Rat
    Clonality
    • 71
    • 40
    • 2
    Monoclonal
    Conjugate
    • 83
    • 9
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This alpha Tubulin antibody is un-conjugated
    Application
    • 95
    • 44
    • 36
    • 35
    • 33
    • 31
    • 26
    • 15
    • 9
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)
    Brand
    IHC-plus™
    Specificity
    Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.
    Purification
    Affinity purified
    Immunogen
    Yeast tubulin.

    Type of Immunogen: Purified protein
    Clone
    YL1-2
    Isotype
    IgG2a
    Top Product
    Discover our top product TUBA1 Primary Antibody
  • Application Notes
    Approved: ELISA (1:100 - 1:1000), IHC, IHC-Fr, IHC-P (5 μg/mL), IP, RIA, WB
    Comment

    Target Species of Antibody: Yeast

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS,  pH 7.4, 0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target
    alpha Tubulin (TUBA1)
    Alternative Name
    Alpha Tubulin (TUBA1 Products)
    Pathways
    Microtubule Dynamics
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