Collagen IV antibody

Catalog No. ABIN961130

Product Details anti-Collagen IV Antibody

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Brand: IHC-plus™

Specificity: Human Type IV collagen. Cross-reactivity: Human collagen type I: 1 % Human collagen type II: <0.5 %, Human collagen type III: <0.5 %, Human collagen type IV: 100 %, Human collagen type V: 7 %, Human fibronectin: <0.5 %, Human albumin: <0.5 %, Human immunoglobulin <0.5 %.

Purification: Affinity purified

Immunogen: Human placental collagen type IV.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:3000), IF, IHC, IHC-Fr, IHC-P (1:100)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3654 was determined to be 1:100. Suitable for use in ELISA, Indirect immunofluorescence, indirect immunohistochemistry and Dot Blotting (not suitable for Western Blot). ELISA: 1:3000. Indirect immunofluorescent visualization of collagen type IV on cryostat sections of human tissue: 1:20-1:40. Indirect Immunohistochemistry: 1:20-1:40. fresh frozen or acetone fixed specimens. Dot Blotting: 1:300. not recommended for Western Blots.

Not recommended for: WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, mannitol, dextran, no preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen IV (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody