CPY antibody

Catalog No. ABIN96043

Product Details anti-CPY Antibody

Target: CPY (Carboxypeptidase Y (CPY))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPY antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Carboxypeptidase Y Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Carboxypeptidase Y [Baker's Yeast].

Characteristics: Synonyms: rabbit anti-Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Carboxypeptidase Y [Baker's Yeast]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Carboxypeptidase Y has been tested by western blot and is suitable to be assayed against 1.0 μg of Carboxypeptidase Y [Baker's Yeast] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-CPY antibody (ABIN96043)

Martinez Benitez, Stolz, Becher, Wolf: "Mnl2, a novel component of the ER associated protein degradation pathway." in: Biochemical and biophysical research communications, (2011) (PubMed).

Ohashi, Munro: "Membrane delivery to the yeast autophagosome from the Golgi-endosomal system." in: Molecular biology of the cell, Vol. 21, Issue 22, pp. 3998-4008, (2011) (PubMed).

von Numers, Survila, Aalto, Heino, Palva, Li: "Requirement of a Homolog of Glucosidase II {beta}-Subunit for EFR-Mediated Defense Signaling in Arabidopsis thaliana." in: Molecular plant, (2010) (PubMed).

Target Details for CPY

Target: CPY (Carboxypeptidase Y (CPY))

Alternative Name: Carboxypeptidase Y (CPY Products)

Background: Background: Carboxypeptidase Y is involved in degradation of small peptides. It digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate. Carboxypeptidase that catalyzes the release of a C-terminal amino acid with broad specificity. It is inhibited by ZPCK.

Gene ID: 855343

NCBI Accession: NP_014026

UniProt: P00729