CA2 antibody

Catalog No. ABIN96021

Supplier Product No.: 200-4157-0100

Product Details anti-CA2 Antibody

Target: CA2 (Carbonic Anhydrase II (CA2))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-4157-0100

Supplier: Rockland

Purpose: Carbonic Anhydrase II Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].

Characteristics: Synonyms: rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II

Purification: Anti-Carbonic Anhydrase II Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Carbonic Anhydrase II Antibody has been tested by Western blot and immunohistochemistry. This product is suitable to be assayed against 1.0 μg of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) [code #611-1302] and TMBE [code # TMBE-1000] as a substrate for 30 minutes at room temperature.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: CA2 (CA2 Products)

Background: Background: Carbonic Anhydrase II Antibody is specific for carbonic anhydrase II. Carbonic anhydrases (or carbonate dehydratases) are a family of metalloenzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and Hydrogen (or vice versa). This reversible reaction occurs slowly in the absence of the carbonic anhydrase catalyst. The active site of most carbonic anhydrases contains a zinc ion cofactor. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues.

Gene ID: 280740

NCBI Accession: NP_848667

UniProt: P00921