Collagen IV antibody

Catalog No. ABIN959465

Product Details anti-Collagen IV Antibody

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificity: Recognizes the extracellular, basement membranes.

No Cross-Reactivity: Rabbit, Rat (Rattus)

Purification: Purified

Immunogen: Type IV collagen isolated from human placenta

Clone: 1043

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IHC, IHC-Fr (1:50 - 1:100), WB (1:100 - 1:1000)

Usage: Methanol fixed cells are recommended for immunocytochemistry. A PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 is recommended for immunohistochemistry on frozen tissues. For immunohistochemistry on paraffin tissue sections, the sections should be pretreated with 1 mg/mL pepsin in 0.01 N HCl for 30 minutes at room temperature.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen IV (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody