Keratin 10/13 antibody
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- Target
- Keratin 10/13
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Reactivity
- Human, Cat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- Brand
- IHC-plus™
- Specificity
- Reacts with cytokeratin 10 and 13 in fixed cells and on frozen tissues, but exclusively with cytokeratin 13 in formalin-fixed, paraffin-embedded tissues. Cytokeratin 10 is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas, while cytokeratin 13 is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stratified epithelia, as well as carcinomas derived from these tissues.
- Purification
- Purified
- Immunogen
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Cytoskeletal preparation extracted from human ectocervical epithelium
Type of Immunogen: Protein fraction - Clone
- DE-K13
- Isotype
- IgG2a
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- Application Notes
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Approved: Flo (1:100 - 1:200), ICC, IHC, IHC-Fr (1:100 - 1:200), IHC-P (10 μg/mL), WB (1:100 - 1:1000)
Usage: This antibody will react exclusively with cytokeratin 13 in formalin-fixed, paraffin-embedded tissues. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS containing 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles. Store undiluted.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
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- Target
- Keratin 10/13
- Alternative Name
- Cytokeratin 10+13
- Background
- This antibody detects: Cytokeratin 10+13
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