FITC antibody (Biotin)

Catalog No. ABIN958554

Product Details anti-FITC Antibody (Biotin)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This FITC antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Specificity: FITC. The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. Cross-reactivity This antiserum has not been tested for cross-reactivity with other fluorescent compounds.

Purification: Purified IgG

Immunogen: FITC

Type of Immunogen: Purified protein

Clone: NI 239

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, WB

Usage: This product is intended for use to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e. g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile distilled water 0.5 ml

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC (FITC Products)

Target Type: Chemical

Background: Name/Gene ID: FITC

Synonyms: FITC