Human Complement C4 (C4C). The reactivity of the antiserum is restricted to C4. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C4 protein. Activated C4b may also react with the antiserum. No reaction is obtained with any other plasma protein component or serum. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in double radial immunodiffusion with bovine serum and found negative.
Usage: This product is intended for use in precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of C4 containing complexes in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non-precipitating antibody-binding techniques if proper controls are included.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Sterile distilled water 1 ml
Concentration
Lot specific
Buffer
Lyophilized antiserum
Handling Advice
Avoid repeat freeze-thaw cycles.
Storage
4 °C,-20 °C
Storage Comment
Long term: -20°C Short term: +4°C. Avoid repeat freeze-thaw cycles.