MANF antibody

Catalog No. ABIN956417

Product Details anti-MANF Antibody

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MANF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human MANF

Cross-Reactivity (Details): No cross-reactivity in MANF-knock out mouse and with CDNF in IHC, slightly cross-reacts with purified CDNF in WB

Purification: MANF-affinity purified

Immunogen: Recombinant human MANF protein produced using CHO-basedIcosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purifiedfrom cell culture supernatant

Isotype: IgG

Application Details

Application Notes: ELISA1:200 000, Western immunoblotting 1:2000 – 1:6000, IHC, IF.  Polyclonal antibody working titer has to be established practically for each particularantigen and assay format

Comment:

Synonym: ARMET, ARP

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.45 mg/ml

Buffer: Concentrated ammonium sulphide in PBS pH 7.4.

Handling Advice: As product is (NH4)2SO4precipitate, mix well by pipetting or vortexing prior use.

Storage: 4 °C

Storage Comment: This product is shipped in non-frozen liquid form in ambient conditions

References for anti-MANF antibody (ABIN956417)

Herranen, Ikäheimo, Lankinen, Pakarinen, Fritzsch, Saarma, Lindahl, Pirvola: "Deficiency of the ER-stress-regulator MANF triggers progressive outer hair cell death and hearing loss." in: Cell death & disease, Vol. 11, Issue 2, pp. 100, (2020) (PubMed).

Lindahl, Chalazonitis, Palm, Pakarinen, Danilova, Pham, Setlik, Rao, Võikar, Huotari, Kopra, Andressoo, Piepponen, Airavaara, Panhelainen, Gershon, Saarma: "Cerebral dopamine neurotrophic factor-deficiency leads to degeneration of enteric neurons and altered brain dopamine neuronal function in mice." in: Neurobiology of disease, Vol. 134, pp. 104696, (2020) (PubMed).

Pakarinen, Danilova, Võikar, Chmielarz, Piepponen, Airavaara, Saarma, Lindahl: "MANF Ablation Causes Prolonged Activation of the UPR without Neurodegeneration in the Mouse Midbrain Dopamine System." in: eNeuro, Vol. 7, Issue 1, (2020) (PubMed).

Tseng, Danilova, Domanskyi, Saarma, Lindahl, Airavaara: "MANF Is Essential for Neurite Extension and Neuronal Migration in the Developing Cortex." in: eNeuro, Vol. 4, Issue 5, (2018) (PubMed).

Target Details for MANF

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: MANF (MANF Products)

Background: MANF is a trophic factor for midbrain dopamine neuronsin vivo. It prevents the 6-OHDA-induced degeneration of dopamine neurons in rodent models of Parkinson’sdisease (Lindholm et al., 2008, Voutilainen et al., 2009). When administeredafter 6-OHDA-lesioning it restores the dopaminergic function and prevents degenerationof dopamine neurons in substantia nigra pars compacta

UniProt: P55145