Biotin antibody

Catalog No. ABIN95626

Supplier Product No.: 100-4198

Product Details anti-Biotin Antibody

Target: Biotin

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Biotin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 100-4198

Supplier: Rockland

Purpose: Biotin Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, biotinylated IgG and Biotinylated BSA.

Characteristics: Synonyms: rabbit anti-biotin antibody, rabbit anti biotin

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Native Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Biotin has been tested in ELISA and is suitable for immunoblotting (western or dot blot), immunoprecipitation and most immunological methods requiring high titer and specificity.

Western Blot Dilution: 1:10,000 - 1:25,000

ELISA Dilution: 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 78 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-Biotin antibody (ABIN95626)

Jongsma, Bakker, Cabukusta, Liv, van Elsland, Fermie, Akkermans, Kuijl, van der Zanden, Janssen, Hoogzaad, van der Kant, Wijdeven, Klumperman, Berlin, Neefjes: "SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport." in: The EMBO journal, Vol. 39, Issue 6, pp. e102301, (2020) (PubMed).

Landi, Mari, Kleiser, Wolf, Gretzmeier, Wilhelm, Kiritsi, Thünauer, Geiger, Nyström, Reggiori, Claudinon, Römer: "Pseudomonas aeruginosa lectin LecB impairs keratinocyte fitness by abrogating growth factor signalling." in: Life science alliance, Vol. 2, Issue 6, (2020) (PubMed).

Koerver, Papadopoulos, Liu, Kravic, Rota, Brecht, Veenendaal, Polajnar, Bluemke, Ehrmann, Klumperman, Jäättelä, Behrends, Meyer: "The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage." in: EMBO reports, Vol. 20, Issue 10, pp. e48014, (2019) (PubMed).

Sztal, Zhao, Williams, Oorschot, Parslow, Giousoh, Yuen, Hall, Costin, Ramm, Bird, Busch-Nentwich, Stemple, Currie, Cooper, Laing, Nowak, Bryson-Richardson: "Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function." in: Acta neuropathologica, Vol. 130, Issue 3, pp. 389-406, (2016) (PubMed).

Wolf, Kastner, Lührmann: "Antisense-targeted immuno-EM localization of the pre-mRNA path in the spliceosomal C complex." in: RNA (New York, N.Y.), Vol. 18, Issue 7, pp. 1347-57, (2012) (PubMed).

Jubelin, Taieb, Duda, Hsu, Samba-Louaka, Nobe, Penary, Watrin, Nougayrède, Schulman, Stebbins, Oswald: "Pathogenic bacteria target NEDD8-conjugated cullins to hijack host-cell signaling pathways." in: PLoS pathogens, Vol. 6, Issue 9, pp. e1001128, (2011) (PubMed).

Duda, Borg, Scott, Hunt, Hammel, Schulman: "Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation." in: Cell, Vol. 134, Issue 6, pp. 995-1006, (2008) (PubMed).

Peden, Oorschot, Hesser, Austin, Scheller, Klumperman: "Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins." in: The Journal of cell biology, Vol. 164, Issue 7, pp. 1065-76, (2004) (PubMed).

Target Details for Biotin

Target: Biotin

Abstract: Biotin Products

Target Type: Chemical

Background: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.