Biotin antibody

Catalog No. ABIN95626

Product Details anti-Biotin Antibody

Target: Biotin

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Biotin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Characteristics: Concentration Definition: by Refractometry

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Immunogentype:Native

Application Details

Application Notes: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 78 mg/mL

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Biotin antibody (ABIN95626)

Jongsma, Bakker, Cabukusta, Liv, van Elsland, Fermie, Akkermans, Kuijl, van der Zanden, Janssen, Hoogzaad, van der Kant, Wijdeven, Klumperman, Berlin, Neefjes: "SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport." in: The EMBO journal, Vol. 39, Issue 6, pp. e102301, (2020) (PubMed).

Landi, Mari, Kleiser, Wolf, Gretzmeier, Wilhelm, Kiritsi, Thünauer, Geiger, Nyström, Reggiori, Claudinon, Römer: "Pseudomonas aeruginosa lectin LecB impairs keratinocyte fitness by abrogating growth factor signalling." in: Life science alliance, Vol. 2, Issue 6, (2020) (PubMed).

Koerver, Papadopoulos, Liu, Kravic, Rota, Brecht, Veenendaal, Polajnar, Bluemke, Ehrmann, Klumperman, Jäättelä, Behrends, Meyer: "The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage." in: EMBO reports, Vol. 20, Issue 10, pp. e48014, (2019) (PubMed).

Sztal, Zhao, Williams, Oorschot, Parslow, Giousoh, Yuen, Hall, Costin, Ramm, Bird, Busch-Nentwich, Stemple, Currie, Cooper, Laing, Nowak, Bryson-Richardson: "Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function." in: Acta neuropathologica, Vol. 130, Issue 3, pp. 389-406, (2016) (PubMed).

Wolf, Kastner, Lührmann: "Antisense-targeted immuno-EM localization of the pre-mRNA path in the spliceosomal C complex." in: RNA (New York, N.Y.), Vol. 18, Issue 7, pp. 1347-57, (2012) (PubMed).

Jubelin, Taieb, Duda, Hsu, Samba-Louaka, Nobe, Penary, Watrin, Nougayrède, Schulman, Stebbins, Oswald: "Pathogenic bacteria target NEDD8-conjugated cullins to hijack host-cell signaling pathways." in: PLoS pathogens, Vol. 6, Issue 9, pp. e1001128, (2011) (PubMed).

Duda, Borg, Scott, Hunt, Hammel, Schulman: "Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation." in: Cell, Vol. 134, Issue 6, pp. 995-1006, (2008) (PubMed).

Peden, Oorschot, Hesser, Austin, Scheller, Klumperman: "Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins." in: The Journal of cell biology, Vol. 164, Issue 7, pp. 1065-76, (2004) (PubMed).

Target Details for Biotin

Target: Biotin

Abstract: Biotin Products

Target Type: Chemical