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Vimentin antibody

Name: Vimentin antibody
SKU: ABIN955516
Price: 1044 USD
Availability: InStock

VIM Reactivity: Human, Mouse WB, IF, IHC (p) Host: Chicken Polyclonal unconjugated

Western Blotting (WB) image for anti-Vimentin (VIM) antibody (ABIN955516) (Vimentin antibody)

In the Left panel (a tissue section through an e18 Mouse cerebellum), Vimentin (brown staining) can be seen in immature astrocytes of the white matter, as well as in vascular endothelial cells. (Vimentin antibody)

2 images

Catalog No. ABIN955516

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Reactivity
304

120

116

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Human, Mouse
Host
158

130

10

6

6

5

2
Chicken
Clonality
171

145
Polyclonal
Conjugate
197

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
262

115

104

96

88

63

48

40

39

33

23

20

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity

Recognizes Vimentin.

Purification

Affinity Chromatography using a peptide column.

Immunogen

Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Vimentin gene product, but are shared between the Human (NP_003371) and Mouse (NP_035831) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Isotype

Ig Fraction

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anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Concentration

0.3 mg/mL

Buffer

10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store the antibody undiluted in the dark at 2-8 °C.
Target

Vimentin (VIM)

Alternative Name

Vimentin (VIM Products)

Background

Human vimentin is a 53,521 dalton protein (466 amino acids) expressed in many cell types of mesodermal origin, and is one of the intermediate filament types that form the cytoskeleton. In the embryonic nervous system, however, vimentin is expressed by migrating neural crest cells forming the PNS, and by neural stem cells of the CNS. In some forms of glioblastoma, vimenin is reexpressed by the tumor cells and serves as a general indicator of their undifferentiated state.Synonyms: VIM

Gene ID

7431

NCBI Accession

NP_003371

Pathways

Caspase Cascade in Apoptosis