Aspartate Transaminase antibody

Catalog No. ABIN95406

Supplier Product No.: 200-601-144-0100

Product Details anti-Aspartate Transaminase Antibody

Target: Aspartate Transaminase (Ast)

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This Aspartate Transaminase antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 200-601-144-0100

Supplier: Rockland

Purpose: Aspartate Transaminase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Aspartate Transaminase (AST) [Pig Heart].

Characteristics: Synonyms: sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody, AST

Purification: Anti-Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Aspartate Transaminase antibody was produced by repeated immunizations with Aspartate Transaminase from pig heart.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Aspartate Transaminase is suitable for western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Aspartate Transaminase

Target: Aspartate Transaminase (Ast)

Alternative Name: Aspartate Transaminase (Ast Products)

Background: Background: Anti-Aspartate Transaminase is detects Aspartate Transaminase. Aspartate Transaminase is a pyridoxal phosphate (PLP)-dependent transaminase enzyme. AST catalyzes the reversible transfer of an α-amino group between aspartate and glutamate and, as such, is an important enzyme in amino acid metabolism. Anti-Aspartate Transaminase antibody is ideal for investigators involved in Cell Signaling, cellular biology and Signal Transduction research.

Gene ID: 396967

NCBI Accession: NP_999092

UniProt: P00503