Aspartate Transaminase antibody

Catalog No. ABIN95405

Supplier Product No.: 100-601-144

Product Details anti-Aspartate Transaminase Antibody

Target: Aspartate Transaminase (Ast)

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This Aspartate Transaminase antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 100-601-144

Supplier: Rockland

Purpose: Aspartate Transaminase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].

Characteristics: Synonyms: sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Aspartate Aminotransferase (AST) [Pig Heart]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Aspartate Transaminase has been assayed against 1.0 μg of Aspartate Aminotransferase (AST) [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 96 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Aspartate Transaminase antibody (ABIN95405)

Mikami, Shibuya, Kimura, Nagahara, Yamada, Kimura: "Hydrogen sulfide protects the retina from light-induced degeneration by the modulation of Ca2+ influx." in: The Journal of biological chemistry, (2011) (PubMed).

Target Details for Aspartate Transaminase

Target: Aspartate Transaminase (Ast)

Alternative Name: Aspartate Transaminase (Ast Products)

Background: Background: Aspartate aminotransferase is involved in the biosynthesis of L-glutamate from L-aspartate or L-cysteine. It is an important regulator of the levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. It acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, Aspartate aminotransferase regulates levels of mercaptopyruvate, an important source of hydrogen sulfide, then mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In eukaryotes there are cytoplasmic, mitochondrial, and chloroplastic isozymes.

Gene ID: 396967

NCBI Accession: NP_999092

UniProt: P00503