AnsB antibody
-
- Target
- AnsB
- Reactivity
- E. coli
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
- Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Supplier Product No.
- 100-4171
- Supplier
- Rockland
- Purpose
- L-Asparaginase Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Asparaginase [Escherichia coli].
- Characteristics
- Synonyms: rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody
- Purification
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- Immunogen
-
Immunogen: Asparaginase [Escherichia coli]
Immunogen Type: Native Protein
-
anti-AnsB antibody (HRP)
Reactivity: E. coli ELISA, WB Host: Rabbit Polyclonal HRP
anti-AnsB antibody (Biotin)Reactivity: E. coli ELISA, WB, DB Host: Rabbit Polyclonal Biotin
-
- Application Notes
-
Immunohistochemistry Dilution: User Optimized
Application Note: Anti-L-asparaginase has been tested by western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 65 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None - Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
-
- Target
- AnsB
- Background
- Background: L-asparaginase from E.coli has two isoenzymes: L-asparaginase I, a low-affinity enzyme located in the cytoplasm, and L-asparaginase II, a high-affinity secreted enzyme.
- Gene ID
- 1039460
- UniProt
- P00805
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