Liver Arginase antibody

Catalog No. ABIN95354

Product Details anti-Liver Arginase Antibody

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Liver Arginase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Arginase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Arginase [Bovine Liver].

Characteristics: Synonyms: rabbit anti-Arginase Antibody, A I antibody, Al antibody, ARG 1 antibody, ARG1 antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase1 antibody, Liver type arginase antibody, Type I arginase antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Arginase [Bovine Liver]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Arginase has been tested by ELISA and western blot. This product has been assayed against 1.0 μg of Arginase [Bovine Liver] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:6,000 to 1:80,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: ARG1 (ARG1 Products)

Background: Background: Arginase has a Catalytic activity of L-arginine + H2O = L-ornithine + urea. Arginase is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.

Gene ID: 513608

NCBI Accession: NP_001039619

UniProt: Q2KJ64

Pathways: Cellular Response to Molecule of Bacterial Origin