MAP2 antibody

Catalog No. ABIN953294

Product Details anti-MAP2 Antibody

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivity: Human, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes MAP-2 (Microtubule-Associated Protein-2).

Purification: Affinity Chromatography using a peptide column.

Immunogen: Two synthetic peptides KLH conjugated corresponding to different regions of the MAP-2 gene product, but are shared between the Human (NP_002365) and Mouse (P20357) sequences. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter- sterilized.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted in the dark at 2-8 °C.

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: MAP-2 (MAP2 Products)

Background: Human MAP-2 is a 199,296 dalton protein (1827 amino acids) expressed in neurons of the PNS and CNS, where it serves as a major component of the neuronal cytoskeleton. MAP-2 contributes to structural integrity and cell shape. This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The exact function of this gene is still unknown. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker

Gene ID: 4133

NCBI Accession: NP_002365