ASK1 antibody (pSer83)

Catalog No. ABIN95283

Supplier Product No.: 200-401-410

Product Details anti-ASK1 Antibody

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Binding Specificity: pSer83

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-401-410

Supplier: Rockland

Purpose: ASK1 phospho S83 Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Characteristics: Synonyms: rabbit anti-ASK1 pS83 Antibody, Apoptosis signal regulating kinase 1 antibody, ASK 1 antibody, ASK-1 antibody, MAP/ERK kinase kinase 5 antibody, MAP3K5 antibody, MAPK/ERK kinase kinase 5 antibody

Purification: This product is an IgG fraction antibody purified from antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to an internal region near amino acids 75-100 of human ASK-1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, immunohistochemistry and immunoprecipitation. For immunoblotting a 1:1,000 dilution is recommended.  A 155 kDa band corresponding to human ASK-1 is detected.  Whole cell lysates from SW1353 can be used as a positive control.  For ELISA a 1:5,000 to 1:10,000 dilution is recommended.   Researchers should determine optimal titers for other applications.

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:5,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-ASK1 antibody (ABIN95283)

Subramanyam, Takahashi, Hasegawa, Mohri, Okada: "Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase." in: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6109-17, (2010) (PubMed).

Target Details for ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Alternative Name: MAP3K5 (MAP3K5 Products)

Background: Background: ASK-1 (apoptosis signal-regulating kinase 1 - also referred to as MEK Kinase-5 or MAPKKK5) is a novel serine/threonine MAP kinase kinase kinase (MAPKKK) component of the mitogen -activated protein (MAP) cascade that is activated in response to extracellular stimuli by cytokines, growth factors and environmental stresses and other factors.   Overexpression of ASK-1 induces apoptotic cell death.  ASK-1 is expressed in a variety of human and mouse tissues.  The overall amino acid sequence identity between the mouse and human ASK1 is 91.9 % .  ASK-1 interacts with CDKN1A (also known as p21, WAF1, CIP1).  Please refer to the reference list at the end of this document for further information.

Gene ID: 4217

NCBI Accession: NP_005914

UniProt: Q99683

Pathways: MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response