GPC2 antibody (N-Term)

Catalog No. ABIN952586

Product Details anti-GPC2 Antibody

Target: GPC2 (Glypican 2 (GPC2))

Binding Specificity: AA 136-165, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPC2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Recognizes GPC2 (N-term).

Purification: Protein A column followed by peptide Affinity purification

Immunogen: KLH conjugated synthetic peptide between 136-165 amino acids from the N-terminal region of Human GPC2 (NP_689955.1) Genename: GPC2

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GPC2

Target: GPC2 (Glypican 2 (GPC2))

Alternative Name: GPC2 (GPC2 Products)

Synonyms: 2410016G05Rik antibody, glypican 2 antibody, glypican 2 (cerebroglycan) antibody, GPC2 antibody, Gpc2 antibody

Background: The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a lycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine rich domain (CRD) with 14 invariant cysteine residues, a stalklike region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35-63 % sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54 % sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17-25 % sequence similarity (1-4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).Synonyms: Glypican-2

Gene ID: 221914

NCBI Accession: NP_689955

Pathways: Glycosaminoglycan Metabolic Process