LC3B antibody

Catalog No. ABIN95223

Supplier Product No.: 200-401-439

Product Details anti-LC3B Antibody

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Supplier Product No.: 200-401-439

Supplier: Rockland

Purpose: Apg8 Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Characteristics: Synonyms: rabbit anti-APG8 Antibody, APG-8, APG 8, ATG-8, ATG 8, Rabbit Anti-ATG8 Antibody, Autophagy-related protein 8, Autophagy-related ubiquitin-like modifier ATG8, Cytoplasm to vacuole targeting protein 5, AUT7, CVT5, YBL078C, YBL0732

Purification: Anti-Apg8 antibody, also known as Atg8, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Rabbit Anti-Apg8 antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Apg8 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Apg8 purified polyclonal antibody reacts with yeast APG8 by western blot and ELISA. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates).  Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. For immunoblotting a 14 kDa band corresponding to yeast Apg8 is detected.  Most yeast cell lysates can be used as a positive control without induction or stimulation.  Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Western Blot Dilution: 1:4,000 - 1:8,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: ATG8 (MAP1LC3B Products)

Background: Background: Anti-Atg8 antibody is an Ubiquitin-like protein (UBL) antibody. UBL proteins and antibodies fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  Apg8 is required for autophagy (intracellular bulk protein degradation) in yeast.  Starved yeast cells take up their own cytoplasm into vacuoles through autophagic bodies.   Autophagic bodies form a double-membraned structure called the autophagosome, which subsequently fuses with the vacuole/lysosome. This process similar in mammals.  Two sets of genes, APG and AUT, have been identified with this process, and are responsible for two ubiquitin-like systems Apg12 and Apg8, respectively.  Apg12 is synthesized in its mature form and seems to have one target, Apg5.  Almost all Apg12 Molecules are conjugated with Apg5.  Aut2/Apg4 processes the Apg8/Aut7 system at its carboxy-terminal region.  Apg8 exists in two forms, one is membrane bound through a phospholipid. Lipidation/activation of Apg8 is mediated by Apg7 and transferred to Apg3 and finally forms a conjugate with phosphatidyl-ethanolamine (PE). Apg4 cleaves Apg8-PE, releasing Apg8 from membrane.  Morphological studies show that Apg8 localizes on the membrane of intermediate structures of the autophagosome, this transient association seems to be essential for formation of the autophagosome. Both Apg12 and Apg8 are highly conserved, with apparent homologues in the worm, mammals and plants. In higher eukaryotes, Apg8 consists of a multigene family. Anti-ATG8 Antibody is useful for researcher interested in autophagy, Cell Biology, Microbiology and Signal Transduction research.

Gene ID: 852200, 6319393

UniProt: P38182

Pathways: Autophagy