CD1e antibody (Middle Region)

Catalog No. ABIN951216

Product Details anti-CD1e Antibody

Target: CD1e

Binding Specificity: AA 274-304, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD1e antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to CD1E.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human CD1E

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1000. Western blot: 1/100 - 1/500. Immunohistochemistry on paraffin sections: 1/50 - 1/100. Flow Cytometry: 1/10 - 1/50. Immunofluorescence: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD1e

Target: CD1e

Alternative Name: CD1E (CD1e Products)

Synonyms: CD1A antibody, R2 antibody, CD1-e antibody, CD1e molecule antibody, CD1E antibody, Cd1e antibody

Background: CD1E encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members.Synonyms: CD_antigen=CD1e, R2G1, T-cell surface glycoprotein CD1e, hCD1e, membrane-associated, sCD1e, soluble

Molecular Weight: 43626 Da

Gene ID: 913

NCBI Accession: NP_001036048