CTLA4 antibody (N-Term)

Catalog No. ABIN951196

Product Details anti-CTLA4 Antibody

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Binding Specificity: AA 57-86, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes Human CD152 / CTLA4 (N-term).

Purification: Affinity Chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 57-86 amino acids from the N-terminal region of Human CD152 / CTLA4. Genename: CTLA4

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CTLA4 antibody (ABIN951196)

Karpathiou, Chauleur, Hathroubi, Habougit, Peoch: "Expression of CD3, PD-L1 and CTLA-4 in mammary and extra-mammary Paget disease." in: Cancer immunology, immunotherapy : CII, Vol. 67, Issue 8, pp. 1297-1303, (2018) (PubMed).

Karpathiou, Casteillo, Giroult, Forest, Fournel, Monaya, Froudarakis, Dumollard, Prades, Peoch: "Prognostic impact of immune microenvironment in laryngeal and pharyngeal squamous cell carcinoma: Immune cell subtypes, immuno-suppressive pathways and clinicopathologic characteristics." in: Oncotarget, Vol. 8, Issue 12, pp. 19310-19322, (2017) (PubMed).

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CD152 / CTLA4 (CTLA4 Products)

Background: CTLA4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.Synonyms: CTLA-4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4

Molecular Weight: 24656 Da

Gene ID: 1493

NCBI Accession: NP_001032720

Pathways: Cancer Immune Checkpoints